How do you prepare a quercetin standard curve?

2.5. For total flavonoid determination, quercetin was used to make the standard calibration curve. Stock quercetin solution was prepared by dissolving 5.0 mg quercetin in 1.0 mL methanol, then the standard solutions of quercetin were prepared by serial dilutions using methanol (5–200 μg/mL).

How do you calculate quercetin?

The Total Flavonoid Content (TFC) for each sample variation can be calculated using Equation 1 as miligrams of Quercetin Equivalent (QE) per gram of anti-atherosclerotic herbs by multiplying the quercetin concentration of the sample in ppm (C) by the volume of the solution in mL (V), and then dividing it by herb mass …

How do you make a standard curve of gallic acid?

To prepare a calibration curve, add 0, 1, 2, 3, 5, and 10 mL of the above phenol stock solution into 100 mL volumetric flasks, and then dilute to volume with water. These solutions will have phenol concentrations of 0, 50, 100, 150, 250, and 500 mg/L gallic acid, the effective range of the assay.

How is total flavonoid content calculated?

The traditional methods for determining total flavonoids in botanical materials are based on chemical extraction and couple with various analytical techniques, such as HPLC [13–15], GC [16], and ultraviolet spectrometry [17].

What color is quercetin?

[14] It is yellow color and is poorly soluble in hot water, quite soluble in alcohol and lipids and is insoluble in cold water. Quercetin is said to be one of the most widely used bioflavonoids for the treatment of metabolic and inflammatory disorders.

What is quercetin made out of?

Quercetin is a plant pigment (flavonoid). It’s found in many plants and foods, such as red wine, onions, green tea, apples, and berries.

Why gallic acid is used as standard in total phenolic content?

I found the details of Folin-Ciocalteu reaction, and the exact reason of choosing Gallic acid as a standard… the reasons are: Partly for historical reasons, In addition it is inexpensive, soluble in water, recrystallized easily from water, readily dried, and stable in the dry form.

Is Quercetin a flavonoid?

Quercetin belongs to a group of plant pigments called flavonoids that give many fruits, flowers, and vegetables their colors. Flavonoids, such as quercetin, are antioxidants.

Can you use 70-80% ethanol in quercetin solution?

While making standard Quercetin solution for the determination of flavonoids, it is dissolved in 70-80% ethanol. Can we use absolute ethanol so that we can get maximum solubility of quercetin in our solvent. What are the drawbacks/problem in using absolute ethanol in this case. Also, Quercetin doesn’t seems to be soluble properly in 70-80% ethanol.

What is the melting point of quercetin in HPLC?

Melting point of standard quercetin found to be 316°C (Literature value: 316°C).11 Several mobile phase compositions were tried and a satisfactory separation and good peak symmetry were obtained by using the mobile phase composition methanol: 0.1% ortho phosphoric acid, (65:35 v/v).

How to determine the presence of quercetin in methanol stem extract?

TLC was done to confirm the presence of quercetin and HPTLC method has been developed for quantification of quercetin in the methanol stem extract. TLC silica gel 60 F 254 plate was used as stationary phase and the solvent system toluene: ethyl acetate: formic acid: methanol (5.5:3:1:0.5) as the mobile phase.

How is quercetin used in the treatment of cancer?

Quercetin is a mitochondrial ATPase and phosphodiesterase inhibitor. It Inhibits PI3-kinase activity and slightly inhibits PIP kinase activity. Quercetin has antiproliferative effects on cancer cell lines, reduces cancer cell growth via type II estrogen receptors, and arrests human leukemic T cells in late G1 phase of the cell cycle.

How do you prepare a quercetin standard curve? 2.5. For total flavonoid determination, quercetin was used to make the standard calibration curve. Stock quercetin solution was prepared by dissolving 5.0 mg quercetin in 1.0 mL methanol, then the standard solutions of quercetin were prepared by serial dilutions using methanol (5–200 μg/mL). How do you calculate…