What is the role of antisense RNA in the plasmid?

Antisense RNAs work through a negative control circuit. They are constitutively synthesized and metabolically unstable. They act both as a measuring device and a regulator, and regulation occurs by inhibition.

How is copy number of ColE1 plasmids controlled?

ColE1 derived plasmids: Antisense RNA In ColE1 derived plasmids, replication is primarily regulated through a small plasmid-encoded RNA called RNA I. More RNA I is produced when the concentration of the plasmid is high, and high concentration of RNA I inhibits replication, resulting in regulation of copy number.

What is the ColE1 origin?

ColE1 is a plasmid found in bacteria. Its name derives from the fact that it carries a gene for colicin E1 (the cea gene). In addition, the plasmid has a series of mobility (mob) genes. Its size and the presence of a single EcoRI recognition site caused it to be considered as a vector candidate.

Which gene is responsible for replication of plasmid?

This plasmid replicates by the theta mechanism, and its replication is independent of DNA Pol I and of a Rep initiator protein. Several palindromes flanking a putative dnaA box are located within the origin of replication of pLS20.

Is RNA sense or antisense?

The sense strand has the information that would be readable on the RNA, and that’s called the coding side. The antisense is the non-coding strand, but ironically, when you’re making RNA, the proteins that are involved in making RNA read the antisense strand in order to create a sense strand for the mRNA.

What is the difference between antisense and RNAi?

Antisense therapy means the selective, sequence-specific inhibition of gene expression by single-stranded DNA oligonucleotides. In contrast, RNA interference (RNAi) is triggered by double-stranded RNA (dsRNA) and causes sequence-specific mRNA degradation of single-stranded target RNAs in response to dsRNA.

What affects plasmid copy number?

Plasmids vary widely in copy number depending on three main factors: 1) The ori and its constituents – (e.g. ColE1 RNA I and RNA II). 2) The size of the plasmid and its associated insert (bigger inserts and plasmids may be replicated at a lower number as they represent a great metabolic burden for the cell).

What is the relationship between plasmid size copy number and DNA yield?

In general, the larger the size of the insert, the lower the yield of plasmid DNA from a given culture medium.

How does a plasmid replicate?

Plasmids utilize their host cell’s replication machinery in order to replicate. As described in our previous Origin of Replication post, DNA replication is initiated at the ORI and may be synchronized with the replication of the host cell’s chromosomal DNA or may be independent of the host’s cell cycle.

How does plasmid replication occur?

Can a plasmid replicate?

The plasmid is a small DNA molecule within a chamber that is physically separated from chromosomal DNA and can replicate independently [6].

What is the role of antisense RNA in the plasmid? Antisense RNAs work through a negative control circuit. They are constitutively synthesized and metabolically unstable. They act both as a measuring device and a regulator, and regulation occurs by inhibition. How is copy number of ColE1 plasmids controlled? ColE1 derived plasmids: Antisense RNA In ColE1…