Why are six-port valves used for injecting samples in HPLC?

These multiple port systems allow un-interrupted flow to the column during the loading of a sample on a sample loop and during injection of a sample under high pressure. As with a dual reciprocating HPLC pump, it is easier to show how a six-port valves works through an animation.

How does a 6 port valve work?

6-Port Column Isolation Valve also isolates the molecules on column and allows components that would adsorb onto the column to bypass the column to the detector. Once heavy components have migrated past the column the valve can be switched OFF and allow components retained on the column to elute.

What is a 6 port valve?

The SV601 series 6 port ball valve is a combination valve body casting for connecting heating and cooling 4 pipe system into a single coil. The valve body design allows a leak free separation from a heating and cooling system and prevents mixture of return flow.

How does HPLC autosampler work?

Autosampler: A device that automates the introduction of an aliquot of sample solution to the HPLC column. An HPLC autosampler typically comprises a sample storage compartment with an injector, consisting of a valve, a sample dosing or metering device, and a moving sampling needle.

What is Loop in HPLC?

The injection loop is a critical component of an HPLC and is potentially one of the largest sources of excess HPLC system volume. The sample is injected into the loop while the loop is switched out of the HPLC flow path. Often when the sample is limited an under fill of the sample loop is performed.

What should we do first before using the HPLC instrument?

What should we do first before using the HPLC instrument? Before the freshly prepare mobile phase is pumped around the HPLC system, it should be thoroughly degassed to remove all dissolved gasses.

Why detectors are used in HPLC?

HPLC detectors are used in the detection of the solute present in the eluent coming from the HPLC column. They are capable of determining the identity and concentration of eluting compounds in the mobile phase. It should have either specific or general response to compounds in a mixture.

What is the basic principle involved in HPLC?

The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). Hence, different constituents of a sample are eluted at different times. Thereby, the separation of the sample ingredients is achieved.

How do I flush my HPLC system?

Flush the column with appropriate solvent found in the column manual. Remove and seal column, and store according to good laboratory practice if needed. Flush system with water to remove buffer. Remove all samples from the sampler and store according to good laboratory practice.

How do I clean my HPLC?

In your case wash the column with 70% water; 15% methanol; 15% acetonitrile. Divert the column eluent to waste not to contaminate your detector(s). Wash the column slowly over to 100% methanol and wash for at least 15 minutes. Wash the column over to 100% acetonitrile and wash for at least 15 minutes.

Why are 6 way valves used in chromatography?

In High Presure Liquid Chromatography (HPLC) the 6 way injection valve allows the introduction of a test sample into a blind loop that is isolated from the flowing high pressure solvent (mobile phase) used to move the sample unto a down stream separation column (stationary phase) .

What kind of valve is used in HPLC?

The six-port valve is the workhorse of HPLC injections. This brilliant invention allows the chromatographer to ‘inject’ a low-pressure sample into a high-pressure mobile phase.

How does sample injection work with a Valco 6 port valve?

Sample Injection with a Valco 6 port valve With the valve in Position A, sample flows through the external loop while the carrier flows directly through to the chromatographic column. When the valve is switched to Position B, the sample contained in the sample loop and valve flow passage is injected onto the column.

Why are six-port valves used for injecting samples in HPLC? These multiple port systems allow un-interrupted flow to the column during the loading of a sample on a sample loop and during injection of a sample under high pressure. As with a dual reciprocating HPLC pump, it is easier to show how a six-port valves…