What media is used for Bordetella pertussis?

The causative agent of the respiratory disease whooping cough, Bordetella pertussis, is a nutritionally fastidious microorganism but can be grown with relative ease in research laboratories. Stainer-Scholte synthetic broth medium and Bordet-Gengou blood agar both support growth of B. pertussis and are commonly used.

What is the best specimen to detect Bordetella pertussis?

Specimen Collection. Determining who has pertussis can be difficult. Whenever possible, clinicians should obtain a nasopharyngeal (NP) swab or aspirate from all persons with suspected cases. A properly obtained NP swab or aspirate is essential for optimal results (see Figures 1 & 2).

Is Bordetella normal flora?

A small, aerobic, gram-negative bacillus which is part of normal respiratory flora of domestic mammals—e.g., dogs, cats—but not humans.

What are the four methods used to identify Bordetella pertussis?

Common laboratory diagnostic methods used for pertussis diagnosis include culture, direct-fluorescent-antibody testing (DFA), serology and polymerase chain reaction (PCR). Culture of Bordetella pertussis is highly specific but fastidious and has limited sensitivity.

Why is it difficult to grow Bordetella pertussis in a laboratory?

Bordetella pertussis is difficult to grow in laboratory because of its inability to grow in agar medium.

How do I know if I have Bordetella?

Clinicians commonly use several types of laboratory tests to diagnose Bordetella pertussis. Scientists consider culture the gold standard because it is the only 100% specific method for identification. Other tests that can be performed include polymerase chain reaction (PCR) and serology.

What are the two types of normal flora?

Although there are many different species of normal flora, these microbes typically fall into one of two categories: 1. resident microbes & 2. transient microbes.

What are the disadvantages of normal flora?

Sometimes normal flora can cause disease in the host body. If somehow the normal defense system or immune system of a host body is unable to restrict or tolerate the normal flora then they can cause diseases in the host body.

Is there a titer test for pertussis?

A Tdap titer is a blood test that checks for immunity from prior vaccination or infection to Tetanus, Diphtheria and Pertussis.

How contagious is Bordetella?

Occasionally, the infection is only caused by a bacterium called Bordetella bronchiseptica. Dogs with this strain of kennel cough can be contagious for between six and 14 weeks.

What’s the best temperature for Bordetella to grow?

Bordetella spp. are minute GNCB. All nine species in the genus are strict aerobes, except for Bordetella petrii, and grow optimally at 35–37°C. Bordetella pertussis and B. parapertussis are nonmotile.

Which is the most common infection caused by Bordetella?

Whooping cough, or pertussis, is the most common human infection caused by the genus Bordetella. It is an acute respiratory infection, affecting primarily non- or under-immunized individuals, especially infants and children. Pertussis is highly communicable, typically by airborne droplets.

How are CD4 + T cells used to detect Bordetella?

The ability to detect Bordetella -specific CD4 + T cell clones in humans recovering from pertussis suggests the possibility that, while antibody may suffice to neutralize the effects of secreted toxins, activation of phagocytic cells may be required for eliminating bacteria from the respiratory tract.

What kind of gene does Bordetella parapertussis have?

B. pertussis and B. parapertussis are nonmotile and all Bordetella spp. share a high degree of DNA homology among virulence genes. Only B. pertussis expresses PT, the major virulence protein.

What media is used for Bordetella pertussis? The causative agent of the respiratory disease whooping cough, Bordetella pertussis, is a nutritionally fastidious microorganism but can be grown with relative ease in research laboratories. Stainer-Scholte synthetic broth medium and Bordet-Gengou blood agar both support growth of B. pertussis and are commonly used. What is the best…